Authors: prof. dr. sc. Marija Vuković Domanovac and dr. sc. Monika Šabić Runjavec
Faculty: University of Zagreb, Faculty of Chemical Engineering and Technology
An autochthonous bacterial culture genus Pseudomonas was isolated from an industrial waste stream with a metabolism adapted to the presence of xenobiotics, making it optimal for remediation. The bacterial isolate was identified based on biochemical tests, the proteins it contained, and gene sequencing. For the cultivation of the isolate, a culture medium was optimised and enriched with 0.1 vol% xenobiotic. Bioagument activated sludge with a bacterial isolate of 0.22 g/L improves bioremediation by reducing the treatment time of industrial wastewater by 6 hours and increasing efficiency by 24.9%. The bacterial isolate showed resistance to stress caused by 26.3% organic load variability, which has manifested by maintaining desired morphological characteristics and abundance under toxic and very toxic conditions. The catabolic potential of the adapted bacterial isolate of 40.3% was expressed in the presence of complex xenobiotics under environmental conditions with bioaugmentation.