Procedure for manufacturing of a miniaturized nanocomposite based multisensing platform for the monitoring of clinically relevant biomarkers

The invention describes the process for obtaining a miniaturized electrochemical multisensing platform based on an innovative electrocatalytic nanomaterial Fullerenol-Hydrogel for the selective determination of clinically relevant markers, such as glucose, lactate, cortisol and H2O2. The electrosensitive layer consists in the simple deposition onto the active surface of a multisensing carbon paste screen-printed electrode (SPE) of an innovative nanocomposite material based on a 3D elastic crosslinked hydrogel network (Hy), doped with conductive polyhydroxylated derivatives of fullerene (fullerenol, FL) containing specific bioreceptors (e.g. enzymes, aptamers). Advantages of fullerenol-hydrogel – based (bio)sensors: enhanced analytical performances for clinical applications, fast response times and low costs, low sample volumes, sensitive, low potential (0.04 V vs. Ag/AgCl) detection of H2O2 using FL-PB-Hydrogel based SPE, sensitive and selective detection of glucose and lactate with FL-PB-Enzymes-Hydrogel based SPE, miniaturization and integration into portable detection systems.
Acknowledgements:

This work is supported by the Ministry of Research, Innovation and Digitization, CNCS/CCCDI – UEFISCDI, project COFUND-M-ERANET-3-FULSENS-GEL within PNCDI III, project no. 318/2022. It is also acknowledged the support of Ministry of Research, Innovation and Digitization through Program 1 – Development of the national research-development system, Subprogram 1.2-Institutional performance- Projects to finance excellence in RDI, Contract no. 15PFE/2021.